NAD+

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What Is NAD+?

Supplement

NAD+ (nicotinamide adenine dinucleotide) is a critical coenzyme found in all living cells that plays a central role in cellular energy metabolism, mitochondrial function, and DNA repair. NAD+ levels naturally decline with age, and supplementation with precursors like NMN (nicotinamide mononucleotide) or NR (nicotinamide riboside) aims to restore NAD+ levels to support healthy aging, cellular energy production, and metabolic function.

NAD+ Research & Studies

01 The role of NAD+ metabolism and its modulation of mitochondria in aging and disease

Comprehensive review showing NAD+ decline is associated with aging and chronic disorders, and that raising cellular NAD+ through precursors can restore mitochondrial and organismal homeostasis, making NAD+ boosting a promising therapeutic strategy.

View Study (Nature)
02 NAD+ precursor supplementation in human ageing: clinical evidence and challenges

Critical review revealing that while preclinical studies support NAD+ supplementation for healthy aging, human clinical trials have shown limited efficacy, with evidence for age-related NAD+ decline consistently observed only in limited studies.

View Study (Nature)
03 The use of a systems approach to increase NAD+ in human participants

Double-blind randomized crossover trial showing that a multi-targeted NAD+ supplement increased whole blood NAD+ levels, increased SIRT1, reduced inflammatory cytokines, and shifted immunoglobulin glycosylation toward a younger biological age.

View Study (Nature)
04 Chronic nicotinamide riboside supplementation is well-tolerated and elevates NAD+ in healthy middle-aged and older adults

2×6-week randomized, double-blind, placebo-controlled crossover trial demonstrating that chronic NR supplementation is well-tolerated, effectively stimulates NAD+ metabolism in healthy middle-aged and older adults, and may reduce blood pressure and arterial stiffness.

View Study (Nature)

NAD+ User Reviews & Experiences

45% Mixed

*Based on large scale analysis of publicly available user experiences

User experiences are genuinely mixed, with notable research support for NAD+ biology but limited clinical evidence of meaningful benefits in humans. While precursors are well-tolerated and can raise NAD+ levels, the translation to measurable health improvements remains uncertain based on current data.

NAD+ Benefits, Dosage & Side Effects

Effects
  • Mitochondrial Support: NAD+ is essential for mitochondrial energy production and cellular respiration through its role in the electron transport chain
  • Cellular Aging Modulation: NAD+ serves as substrate for sirtuins and other enzymes involved in DNA repair, cellular senescence, and longevity pathways
  • Anti-inflammatory Effects: NAD+ supplementation has been shown to reduce pro-inflammatory cytokines including IL-2 in clinical trials
  • Metabolic Function: NAD+ plays a central role in glycolysis, TCA cycle, and regulating nutrient sensing pathways critical for metabolic health
Effectiveness
  • Bioavailability: NR and NMN precursors effectively increase blood NAD+ levels in human trials, though tissue-specific effects vary significantly
  • Clinical Translation Gap: While rodent studies show promising anti-aging effects, human clinical trials have demonstrated limited efficacy for functional health outcomes
  • Individual Variation: NAD+ metabolism appears highly tissue-specific and individualized, making extrapolation from animal studies challenging
  • Systems Approach: Multi-targeted supplements addressing multiple points in the NAD+ salvage pathway show promise for greater efficacy than single precursors
Dosage & Administration
  • NR Dosing: Clinical trials commonly use 250-1000mg daily of nicotinamide riboside, with 6-week supplementation periods showing NAD+ elevation
  • Chronic Supplementation: Evidence suggests chronic rather than acute supplementation is necessary for sustained NAD+ elevation and potential benefits
  • Precursor Selection: Different NAD+ precursors (NR, NMN, niacin) may have varying tissue distribution and efficacy profiles
  • Timing Considerations: Limited human data on optimal timing, though consistent daily dosing appears standard in clinical research
Side Effects
  • Generally Well-Tolerated: Multiple clinical trials report good tolerability with minimal adverse effects from NAD+ precursor supplementation
  • Limited Long-term Data: Safety data for chronic use beyond several months remains limited in human populations
  • Metabolic Effects: Potential impacts on glucose metabolism and blood pressure noted in some studies, requiring monitoring
  • Individual Responses: Side effect profiles may vary based on baseline NAD+ status and individual metabolic characteristics
Availability & Sourcing
  • Supplement Market: NR and NMN are widely available as dietary supplements, though quality and purity vary significantly between manufacturers
  • Research Stage: While precursors are commercially available, the field is still establishing clinical efficacy and optimal formulations for human use

Related Compounds

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